Ashley Callahan

CallahanAResearch Officer
Bio21 Incubator Building
Room G18
+613 9035 4400
University of Melbourne – Find an Expert


I have been working as a Research Officer that the Bio21 Institute for the past six years with the PEARG group. Dengue fever is a mosquito borne viral disease that is transmitted by the mosquitoes Aedes aegypti and Aedes albopictus. Our research program is investigating the naturally occurring bacterium, Wolbachia which has been artificially introduced into A. aegypti to decrease the ability of this mosquito to transmit dengue viruses between people. My research focuses on high through put screening using several lightcycler assays (HRM and Taqman probe) for species identification and for the detection of Wolbachia and insecticide resistance. I have had the opportunity to complete many field experiments and field collections in Cairns as well as Bali. I am proficient in preparing RADseq libraries and my work involves training many post graduate and international visitors in our laboratory techniques.

I am originally from Newfoundland, Canada and I completed my Master of Science (Environmental Science) and Bachelor of Science (Biology) degrees from Memorial University, Canada. In the past, I have worked as a Research Assistant in marine sciences for several years and focused on marine bacteria and aquatic invasive species.


Hoffmann, A. A., Montgomery, B. L., Popovici, J., Iturbe-Ormaetxe, I., Johnson, P. H., Muzzi, F., … & Cook, H. (2011). Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission. Nature476(7361), 454-457.

Hoffmann, A. A., Iturbe-Ormaetxe, I., Callahan, A. G., Phillips, B. L., Billington, K., Axford, J. K., … & O’Neill, S. L. (2014). Stability of the w Mel Wolbachia infection following invasion into Aedes aegypti populations. PLoS Negl Trop Dis8(9), e3115.

Callahan, A. G., Deibel, D., McKenzie, C. H., Hall, J. R., & Rise, M. L. (2010). Survey of harbours in Newfoundland for indigenous and non-indigenous ascidians and an analysis of their cytochrome c oxidase I gene sequences. Aquatic Invasions5(1), 31-39.

Ritchie, S. A., Townsend, M., Paton, C. J., Callahan, A. G., & Hoffmann, A. A. (2015). Application of w MelPop Wolbachia strain to crash local populations of Aedes aegypti. PLoS Negl Trop Dis9(7), e0003930.

Axford, J. K., Ross, P. A., Yeap, H. L., Callahan, A. G., & Hoffmann, A. A. (2016). Fitness of wAlbB Wolbachia infection in Aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion. The American journal of tropical medicine and hygiene94(3), 507-516.

Rašić, G., Filipović, I., Callahan, A. G., Stanford, D., Chan, A., Lam-Phua, S. G., … & Hoffmann, A. A. (2016). The queenslandensis and the type form of the dengue fever mosquito (Aedes aegypti L.) are genomically indistinguishable. PLOS Negl Trop Dis10(11), e0005096.

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